GenoExplorer™ microRNA System

GenoExplorer™ microRNA Full Kit

GenoExplorer™ microRNA full kit provides a complete and comprehensive miRNA profiling analysis tool. The kit contains a set of GenoExplorer™ microRNA chips, labeling kit, hybridization and washing buffers, and protocols for use, scanning, and data analysis. The kit is flexible and compatible with most hybridization chambers and microarray scanners. The GenoExplorer™ miRNA full kit is simple and easy to use (no enrichment required!), and chips provide updated and comprehensive miRNA information. The whole kit allows you to receive

The updated miRNA sequences with mature and most precursor probes all on one array (miRBase v15), for human over 1500 miRNA probes, mouse and rat over 1200 probes, and more species

Positive and negative control probes (eg. 5S rRNA, tRNAs, U6)

High signal to noise ratio and subfemtamole sensitivity with only 1 µg total RNA input (for optimal sensitivity we recommend using 1-2.5 µg total RNA although 100-200 ng total RNA can generate biologically significant results)

Specificity of up to 94%

Detection of less than 2 fold differential miRNA expression

Highly reproducible results with CV < 15% between experiments

> 3 logs of dynamic range on most scanners (eg. Axon, Agilent)

The most convenient and cost-effective tool to meet your needs

User's manual provides a step-by-step assay protocol

Technical Resources

Assay performance and technical bulletins provide experiment data on product performance

General information about the product kits describes product design and assay performance

Probe list on arrays (for Catalog 1100C's, 1200C's and 1400C's representing Sanger miRBase v15) human, mouse and rat, C.elegans, Drosophila, and Arabidopsis

Probe list on arrays (for Catalog 1100v8's, 1200v8's and 1400v8's representing Sanger miRBase v8.2 and 9.1) human, mouse, rat, C.elegans, Drosophila, Arabidopsis

Gal file is available for array image gridding (for catalog 1100v8’s and 1200v8’s)

human, mouse/rat, C.elegans, Drosophila, and Arabidopsis

General information about full service describes product design and assay performance
Full service data output format demonstrates data output and format

Full service data output format demonstrates data output and format

Selected recent publications (GenoSensor reference):

• Li G et al. Modulation of inflammatory markers by miR-146a during replicative senescence in trabecular meshwork cells. Invest Ophthalmol Vis Sci 2010; Jan 6

• Hanoun N et al. The silencing of microRNA 148a production by DNA hypermethylation is an early event in pancreatic cancinogenesis. Clin Chem 2010; Apr 29

• Liang Z et al. Involvement of miR-326 in chemotherapy resistance of breast cancer. Biochem Pharmacol 2010; 79(6):817-24

• Liu X et al. microRNA-138 suppresses invasion and promotes apoptosis in head and neck squamous cell carcin. Cancer Lett2009; 286(2):217-22

• Lee S-O et al. MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney diseases. J Clin Invest 2008; 18(11):3714-24

• Chang SS et al. miRNA alterations inhead and neck squamous cell carcinoma. Int J Cancer 2008; 123,2791-7

• Park CS and Tang SJ. Regulation of microRNA Expression by Induction of Bidirectional Synaptic Plasticity. J Mol Neurosci. 2008; Nov 8

• Chen X-M et al. A cellular miRNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J. Biol. Chem. 2007; July 27

• Baroukh N etc. MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta -cells lines. J Biol Chem 2007; Apr 26

• Weston MD et al. MicroRNA gene expression in the mouse inner ear.
Brain Res 2006; 1111(1)95-104

Contact us if you want to order or have more questions.

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