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GenoExplorer™ microRNA System

GenoExplorer™ microRNA System

GenoExplorer™ microRNA Quantitative RT-PCR Kit

The GenoExplorer™ miRNA quantitative RT-PCR kit has been developed and optimized to amplify specific microRNAs, allowing real time quantitation of miRNA expression levels. This system can achieve

  • Sensitive detection requires only 1-10ng total RNA or equivalent
  • Specific single nucleotide discrimination
  • Accurate over a broad range of samples
  • Dynamic range >7 orders of magnitude
  • Available for both mature and precursor miRNAs
  • One stop validation of miRNA expression profiling data
  • Endpoint or Real Time PCR
  • Quality, High Performance, Reliability
  • Primer sets available

    • User's manual provides a step-by-step assay protocol

    Cat # Product Name Description Size Price
    2001 GenoExplorer™ miRNA qRT-PCR Kit-100 Provides complete set for miRNA quantitation including first-strand cDNA synthesis and SYBR® Green I qPCR reagent mix. (primers not included, see Cat# 2003 and 2004) 100
    reactions    		 US$260
    2002-10 GenoExplorer™ miRNA First-Strand cDNA Core Kit-10 Provides necessary core components to generate miRNA first-strand cDNA for miRNA expression quantitation. 10
    reactions    		 US$195
    2002-50 GenoExplorer™ miRNA First-Strand cDNA Core Kit-50 Provides necessary core components to generate miRNA first-strand cDNA for miRNA expression quantitation. 50
    reactions    		 US$590
    2003 GenoExplorer™ miRNA qPCR Primer Sets

    Provides specific primer pair for miRNA quantitation analysis (3µM each). Suggest to combine with GenoExplorer™ miRNA qRT-PCR Kit-100 (Cat# 2001) and reference primer sets (Cat# 2004). Refer miRBase for available genes and primers.

    When ordering, enter species-mir-number. e.g. hsa-miR-125a, mmu-mir-124-1-precursor
    		100 µl US$60
    2004 GenoExplorer™ miRNA qPCR Reference Primer Sets

    Reference primer as an internal control reference (3µM each). Suggest to combine with GenoExplorer™ miRNA qRT-PCR Kit-100 (Cat# 2001) and GenoExplorer™ miRNA qPCR Primer Sets (Cat# 2003)

    Selection of human-U6, human-U44, human-5SrRNA; mouse-U6, mouse-5SrRNA; rat-U6, rat-5SrRNA; Drosophila-U6 and 5SrRNA
    		100 µl US$60
    2005 GenoExplorer™ 2X qPCR Mix 2X SYBR® Green I qPCR mix, specifically for miRNA and small RNA amplification and quantification, containing all PCR reagents and polymerase 750 µl US$145

    GenoExplorer™ microRNA qRT-PCR Kit
    The GenoExplorer™ miRNA qRT-PCR kit is designed to quantify miRNA expression levels that include first-strand cDNA synthesis and quantitative PCR steps. The Kit is sufficient for synthesizing 10 cDNA samples and 100 quantitation reactions (Cat # 2001).

    GenoExplorer™ microRNA First-Strand cDNA Core Kit
    The GenoExplorer™ miRNA first-strand core kit is designed to generate first-strand cDNA from microRNAs or small RNAs that contains elongation of miRNA template and reverse transcription steps. The Core Kit is sufficient for either 10 reactions (Cat # 2001-10) or 50 reactions (Cat # 2001-50).

    GenoExplorer™ microRNA qPCR Primer Sets
    GenoExplorer™ miRNA qPCR primer sets are designed for both mature and precursor microRNAs, and for use with the GenoExplorerTM miRNA qRT-PCR Kit (Cat # 2001), with which the primer sets can be used for 100 PCR reactions. Primer Sets are available for human, mouse, rat, and other species. Refer miRBase for available genes and primers or contact us for details.

    Technical Resources

    MicroRNA qRT-PCR assay performance and technical bulletins provide experiment data on product performance (link qrt-pcr Tech Performance doc)

    Primers for both mature and precursor miRNAs are available (refer Senger sequence reference)

    Related Products

    GenoExplorer™ microRNA array full kit provides a complete and easy-to-use kit with selective labeling procedure using total RNA (no enrichment required!) combined with a highly sensitive and reproducible assay. The arrays contain not only mature but precursor probes as well.

    GenoExplorer™ microRNA array labeling kit directly labels RNA molecules resulting in a highly effective and selective labeling method (no enrichment required!) for both animals and plants.

    GenoExplorer™ microRNA array chip is composed of mature and precursor miRNA sequences and probes that covalently bind onto a 3-D matrix slide.

    GenoExplorer™ microRNA array probe set contains both mature and precursor miRNA sequences as well as multiple positive and negative controls.

    Other reagents related to miRNA expression analysis include hybridization buffer, washing solutions, and probe printing buffer.

    Selected Recent Publications (GenoSensor Reference):

    • Park JH et al. Murine hepatic miRNAs expression and regulation of gene expression in diet-induced obese mice. Mol. Cells 2011; 31:33-38.

    • Foss KM et al. miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. J Thoracic Oncol 2011; 6(3):482-8.

    • Arora S et al. MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. Intl J Cancer 2011; 128(11): Mar 29.

    • Leeper NJ et al. MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cellular Physiol 2011; 226(4):1035-43.

    • Lu C and Souret F. High-throughput approaches for miRNA expression analysis. Plant MicroRNAs 2010; 592:107-25.

    • Mouillet JF et al. MiR-205 silences MED1 in hypoxic primary human trophoblasts. FASEB J 2010; 24(6):2030-9.

    • Ranade AR et al. MicroRNA 92a-2*: a biomarker predictive for chemoresistance and prognostic for survival in patients with small cell lung cancer. J Thoracic Oncol 2010; 5(8):1273-8.

    • Li G et al. Modulation of inflammatory markers by miR-146a during replicative senescence in trabecular meshwork cells. Invest Ophthalmol Vis Sci 2010; Jan 6

    • Hanoun N et al. The silencing of microRNA 148a production by DNA hypermethylation is an early event in pancreatic cancinogenesis. Clin Chem 2010; Apr 29

    • Liang Z et al. Involvement of miR-326 in chemotherapy resistance of breast cancer. Biochem Pharmacol 2010; 79(6):817-24

    • Igoucheva O and Alexeev V MicroRNA-dependent regulation of cKit in cutaneous melanoma. Biochem Biophys Res Comm 2009; 379(3):790-4.

    • Liu X et al. MicroRNA-222 regulates cell invasion by targeting matrix metalloproteinase 1 (MMP1) and manganese superoxide dismutase 2 (SOD2) in tongue squamous cell carcinoma cell lines. Cancer Genomics Proteomics 2009; 6(3):131-9.

    • Nasser S et al. Identifying miRNA and imaging features associated with metastasis of lung cancer to the brain. IEEE 2009

    • Blow N. Small RNAs: biology’s brave new world. Nature Methods 2009; 6(3):232-5.

    • Liu X et al. microRNA-138 suppresses invasion and promotes apoptosis in head and neck squamous cell carcin. Cancer Lett2009; 286(2):217-22

    • Lee S-O et al. MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney diseases. J Clin Invest 2008; 18(11):3714-24

    • Chang SS et al. miRNA alterations inhead and neck squamous cell carcinoma. Int J Cancer 2008; 123,2791-7

    • Park CS and Tang SJ. Regulation of microRNA Expression by Induction of Bidirectional Synaptic Plasticity. J Mol Neurosci. 2008; Nov 8

    • Goff LA et al. The analysis of microRNAs in stem cells. Stem Cell Res Therap 2008; 1:141-67.

    • Chang HY et al. Microarray analysis of stem cells and their differentiation. Essential stem cell methods 2008; Chapter 12.

    • Chen X-M et al. A cellular miRNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J. Biol. Chem. 2007; July 27

    • Baroukh N etc. MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta -cells lines. J Biol Chem 2007; Apr 26

    • Donker RB et al. The expression of argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Mol Hum Reprod 2007; 13(4):273-9.

    • Mott JL et al. Mir-29 regulates Mcl-1 protein expression and apoptosismer-29 downregulates Mcl-1. Oncogene 2007; 26:6133-40.

    • Weston MD et al. MicroRNA gene expression in the mouse inner ear.
    Brain Res 2006; 1111(1)95-104

    Contact us if you want to order or have more questions.
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