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GenoExplorer™ microRNA System

GenoExplorer™ microRNA System

GenoExplorer™ microRNA Quantitative RT-PCR Kit

The GenoExplorer™ miRNA quantitative RT-PCR kit has been developed and optimized to amplify specific microRNAs, allowing real time quantitation of miRNA expression levels. This system can achieve

  • Sensitive detection requires only 1-10ng total RNA or equivalent
  • Specific single nucleotide discrimination
  • Accurate over a broad range of samples
  • Dynamic range >7 orders of magnitude
  • Available for both mature and precursor miRNAs
  • One stop validation of miRNA expression profiling data
  • Endpoint or Real Time PCR
  • Quality, High Performance, Reliability
  • Primer sets available
  • User's manual provides a step-by-step assay protocol

    Cat # Product Name Description Size Price
    2001 GenoExplorer™ miRNA qRT-PCR Kit-100 Provides complete set for miRNA quantitation including first-strand cDNA synthesis and SYBR® Green I qPCR reagent mix. (primers not included, see Cat# 2003 and 2004) 100
    reactions
    US$260
    2002-10 GenoExplorer™ miRNA First-Strand cDNA Core Kit-10 Provides necessary core components to generate miRNA first-strand cDNA for miRNA expression quantitation. 10
    reactions
    US$195
    2002-50 GenoExplorer™ miRNA First-Strand cDNA Core Kit-50 Provides necessary core components to generate miRNA first-strand cDNA for miRNA expression quantitation. 50
    reactions
    US$310
    2003 GenoExplorer™ miRNA qPCR Primer Sets Provides specific primer pair for miRNA quantitation analysis (3µM each). Suggest to combine with GenoExplorer™ miRNA qRT-PCR Kit-100 (Cat# 2001) and reference primer sets (Cat# 2004) 100 µl US$60
    2004 GenoExplorer™ miRNA qPCR Reference Primer Sets (selection of human-U6, human-U44, mouse-U6, and mouse-5SrRNA) Reference primer as an internal control reference (3µM each). Suggest to combine with GenoExplorer™ miRNA qRT-PCR Kit-100 (Cat# 2001) and GenoExplorer™ miRNA qPCR Primer Sets (Cat# 2003) 100 µl US$60

    GenoExplorer™ microRNA qRT-PCR Kit
    The GenoExplorer™ miRNA qRT-PCR kit is designed to quantify miRNA expression levels that include first-strand cDNA synthesis and quantitative PCR steps. The Kit is sufficient for synthesizing 10 cDNA samples and 100 quantitation reactions (Cat # 2001).

    GenoExplorer™ microRNA First-Strand cDNA Core Kit
    The GenoExplorer™ miRNA first-strand core kit is designed to generate first-strand cDNA from microRNAs or small RNAs that contains elongation of miRNA template and reverse transcription steps. The Core Kit is sufficient for either 10 reactions (Cat # 2001-10) or 50 reactions (Cat # 2001-50).

    GenoExplorer™ microRNA qPCR Primer Sets
    GenoExplorer™ miRNA qPCR primer sets are designed for both mature and precursor microRNAs, and for use with the GenoExplorerTM miRNA qRT-PCR Kit (Cat # 2001), with which the primer sets can be used for 100 PCR reactions. Primer Sets are available for human, mouse, rat, and other species. Refer GenoExplorerTM miRNA array probe list for availability or contact us for details.

    Technical Resources

    MicroRNA qRT-PCR assay performance and technical bulletins provide experiment data on product performance (link qrt-pcr Tech Performance doc)

    Primers for both mature and precursor miRNAs are available (refer Senger sequence reference)

    Contact us if you want to order or have more questions.

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