GenoExplorer™ microRNA System
 GenoExplorer™ microRNA
Quantitative RT-PCR Kit
The GenoExplorer™ miRNA quantitative RT-PCR kit has
been developed and optimized to amplify specific microRNAs,
allowing real time quantitation of miRNA expression levels.
This system can achieve
Sensitive detection requires only 1-10ng total RNA or equivalent
Specific single nucleotide discrimination
Accurate over a broad range of samples
Dynamic range >7 orders of magnitude
Available for both mature and precursor miRNAs
One stop validation of miRNA expression profiling data
Endpoint or Real Time PCR
Quality, High Performance, Reliability
Primer sets available
User's
manual provides a step-by-step assay protocol
| Cat # |
Product Name |
Description |
Size |
Price |
| 2001 |
GenoExplorer™ miRNA qRT-PCR Kit-100 |
Provides complete set for miRNA quantitation
including first-strand cDNA synthesis and SYBR® Green
I qPCR reagent mix. (primers not included, see Cat# 2003
and 2004) |
100
reactions |
US$260 |
| 2002-10 |
GenoExplorer™ miRNA First-Strand cDNA
Core Kit-10 |
Provides necessary core components to generate
miRNA first-strand cDNA for miRNA expression quantitation.
|
10
reactions |
US$195 |
| 2002-50 |
GenoExplorer™ miRNA First-Strand cDNA Core Kit-50 |
Provides necessary core components to generate miRNA
first-strand cDNA for miRNA expression quantitation. |
50
reactions |
US$590 |
| 2003 |
GenoExplorer™ miRNA qPCR Primer Sets |
Provides specific primer pair for miRNA quantitation
analysis (3µM each). Suggest to combine with GenoExplorer™
miRNA qRT-PCR Kit-100 (Cat# 2001) and reference primer
sets (Cat# 2004). Refer miRBase
for available genes and primers.
When ordering, enter species-mir-number. e.g.
hsa-miR-125a, mmu-mir-124-1-precursor
|
|
100 µl |
US$60 |
| 2004 |
GenoExplorer™ miRNA qPCR Reference Primer Sets
|
Reference primer as an internal control reference
(3µM each). Suggest to combine with GenoExplorer™
miRNA qRT-PCR Kit-100 (Cat# 2001) and GenoExplorer™
miRNA qPCR Primer Sets (Cat# 2003)
| Selection of human-U6, human-U44, human-5SrRNA;
mouse-U6, mouse-5SrRNA; rat-U6, rat-5SrRNA |
|
100 µl |
US$60 |
| 2005 |
GenoExplorer™ 2X qPCR Mix |
2X SYBR® Green I qPCR mix, specifically for miRNA
and small RNA amplification and quantification, containing
all PCR reagents and polymerase |
750 µl |
US$145 |
GenoExplorer™ microRNA qRT-PCR Kit
The GenoExplorer™ miRNA qRT-PCR kit is designed to quantify
miRNA expression levels that include first-strand cDNA synthesis
and quantitative PCR steps. The Kit is sufficient for synthesizing
10 cDNA samples and 100 quantitation reactions (Cat # 2001).
GenoExplorer™ microRNA First-Strand cDNA Core
Kit
The GenoExplorer™ miRNA first-strand core kit is designed
to generate first-strand cDNA from microRNAs or small RNAs
that contains elongation of miRNA template and reverse transcription
steps. The Core Kit is sufficient for either 10 reactions
(Cat # 2001-10) or 50 reactions (Cat # 2001-50).
GenoExplorer™ microRNA qPCR Primer Sets
GenoExplorer™ miRNA qPCR primer sets are designed for
both mature and precursor microRNAs, and for use with the
GenoExplorerTM miRNA qRT-PCR Kit (Cat # 2001), with which
the primer sets can be used for 100 PCR reactions. Primer
Sets are available for human, mouse, rat, and other species.
Refer miRBase
for available genes and primers or contact
us for details.
Technical Resources
MicroRNA qRT-PCR assay performance
and technical bulletins provide experiment
data on product performance (link qrt-pcr Tech Performance doc)
Primers for both mature and precursor miRNAs are available
(refer Senger
sequence reference)
Related Products
GenoExplorer™
microRNA array full kit provides a complete and
easy-to-use kit with selective labeling procedure using
total RNA (no enrichment required!) combined with a highly
sensitive and reproducible assay. The arrays contain not
only mature but precursor probes as well.
GenoExplorer™
microRNA array labeling kit directly labels RNA
molecules resulting in a highly effective and selective
labeling method (no enrichment required!) for both animals
and plants.
GenoExplorer™ microRNA
array chip is composed of mature and precursor miRNA
sequences and probes that covalently bind onto a 3-D matrix
slide.
GenoExplorer™
microRNA array probe set contains both mature and
precursor miRNA sequences as well as multiple positive and
negative controls.
Other reagents
related to miRNA expression analysis include hybridization
buffer, washing solutions, and probe printing buffer.
Contact us
if you want to order or have more questions.
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