GenoExplorer™ microRNA System

GenoExplorer™ microRNA Chip

GenoExplorer™ microRNA chips contain the latest updated miRNA sequences. The probes are specifically and functionally validated. Probes were designed based on the full miRNA sequences, computed with their lengths, Tm, and secondary structure. The probes, consisting of mature and precursor sequences, covalently bind onto a 3-D matrix slide. When used with GenoExplorer? microRNA labeling kit, the system provides

The updated miRNA sequences with mature and most precursor probes all on one array (miRBase v14), for human over 1300 miRNA probes, mouse and rat over 1200 probes, and more species

Positive and negative control probes (eg. 5S rRNA, tRNAs, U6)

High signal to noise ratio and subfemtamole sensitivity with 2.5 µg total RNA input (for optimal sensitivity we recommend using 2.5 µg total RNA although 200-300 ng total RNA can generate biologically significant results)

Specificity of up to 94%

Detection of less than 2 fold differential miRNA expression

Highly reproducible results with CV < 15% between experiments

and > 3 logs of dynamic range on most scanners (eg. Axon, Agilent)

User's manual provides a step-by-step assay protocol

Technical Resources

Assay performance and technical bulletins provide experiment data on product performance

General information about the product kits describes product design and assay performance

Probe list on arrays (for Catalog 1100C's, 1200C's and 1400C's representing Sanger miRBase v13.0) human, mouse and rat, C.elegans, Drosophila, and Arabidopsis

Probe list on arrays (for Catalog 1100v8's, 1200v8's and 1400v8's representing Sanger miRBase v8.2 and 9.1) human, mouse, rat, C.elegans, Drosophila, Arabidopsis

Gal file is available for array image gridding (for catalog 1100v8’s and 1200v8’s)

human, mouse/rat, C.elegans, Drosophila, and Arabidopsis

General information about full service describes product design and assay performance
Full service data output format demonstrates data output and format

Full service data output format demonstrates data output and format

Selected recent publications (GenoSensor reference):

• Lee S-O et al. MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney diseases. J Clin Invest 2008; 18(11):3714-24

• Chang SS et al. miRNA alterations inhead and neck squamous cell carcinoma. Int J Cancer 2008; 123,2791-7

• Park CS and Tang SJ. Regulation of microRNA Expression by Induction of Bidirectional Synaptic Plasticity. J Mol Neurosci. 2008; Nov 8

• Chen X-M et al. A cellular miRNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J. Biol. Chem. 2007; July 27

• Baroukh N etc. MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta -cells lines. J Biol Chem 2007; Apr 26

• Weston MD et al. MicroRNA gene expression in the mouse inner ear.
Brain Res 2006; 1111(1)95-104

Contact us if you want to order or have more questions.

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