GenoExplorer™ microRNA System

GenoExplorer™ microRNA Labeling Kit

GenoExplorer™ microRNA labeling kit is designed to label miRNAs and other small RNA molecules for microarray analysis using a simple and highly robust labeling strategy. It directly labels RNA molecules, resulting in a highly effective and selective labeling method (no enrichment required!) for both animals and plants. Combining with GenoExplorer? microRNA arrays, one can achieve

GenoExplorer™ microRNA labeling kit (patented) principle and workflow. Labeled RNA hybridizes with probes on a glass microarray slide.

  • Subfemtamole sensitivity with only 1 µg total RNA input (for optimal sensitivity we recommend using 1-2.5 µg total RNA although 100-200 ng total RNA can generate biologically significant results)
  • Specificity of up to 94%
  • Detection of less than 2 fold differential miRNA expression
  • Uniform labeling and highly reproducible results with CV < 15% between experiments
  • and > 3 logs of dynamic range on most scanners (eg. Axon, Agilent)

  • User's manual provides a step-by-step assay protocol

    Cat # Product Name Description Size Price
    1301 GenoExplorer™microRNA labeling kit GenoExplorer™ microRNA labeling kit 20 reactions US$500
    1302 GenoExplorer™microRNA labeling kit is ONLY available with purchase of full kits 4 reactions N/A

    Technical Resources

    Assay performance and technical bulletins provide experiment data on product performance

    General information about the product kits describes product design and assay performance

    Probe list on arrays (for Catalog 1100C's, 1200C's and 1400C's representing Sanger miRBase)

    General information about full service describes product design and assay performance. Full service data output format demonstrates data output and format

    Full service data output format demonstrates data output and format

    Selected recent publications (GenoSensor reference):

    • Park JH et al. Murine hepatic miRNAs expression and regulation of gene expression in diet-induced obese mice. Mol. Cells 2011; 31:33-38.

    • Foss KM et al. miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. J Thoracic Oncol 2011; 6(3):482-8.

    • Arora S et al. MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. Intl J Cancer 2011; 128(11): Mar 29.

    • Leeper NJ et al. MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cellular Physiol 2011; 226(4):1035-43.

    • Lu C and Souret F. High-throughput approaches for miRNA expression analysis. Plant MicroRNAs 2010; 592:107-25.

    • Mouillet JF et al. MiR-205 silences MED1 in hypoxic primary human trophoblasts. FASEB J 2010; 24(6):2030-9.

    • Ranade AR et al. MicroRNA 92a-2*: a biomarker predictive for chemoresistance and prognostic for survival in patients with small cell lung cancer. J Thoracic Oncol 2010; 5(8):1273-8.

    • Li G et al. Modulation of inflammatory markers by miR-146a during replicative senescence in trabecular meshwork cells. Invest Ophthalmol Vis Sci 2010; Jan 6

    • Hanoun N et al. The silencing of microRNA 148a production by DNA hypermethylation is an early event in pancreatic cancinogenesis. Clin Chem 2010; Apr 29

    • Liang Z et al. Involvement of miR-326 in chemotherapy resistance of breast cancer. Biochem Pharmacol 2010; 79(6):817-24

    • Igoucheva O and Alexeev V MicroRNA-dependent regulation of cKit in cutaneous melanoma. Biochem Biophys Res Comm 2009; 379(3):790-4.

    • Liu X et al. MicroRNA-222 regulates cell invasion by targeting matrix metalloproteinase 1 (MMP1) and manganese superoxide dismutase 2 (SOD2) in tongue squamous cell carcinoma cell lines. Cancer Genomics Proteomics 2009; 6(3):131-9.

    • Nasser S et al. Identifying miRNA and imaging features associated with metastasis of lung cancer to the brain. IEEE 2009

    • Blow N. Small RNAs: biology’s brave new world. Nature Methods 2009; 6(3):232-5.

    • Liu X et al. microRNA-138 suppresses invasion and promotes apoptosis in head and neck squamous cell carcin. Cancer Lett2009; 286(2):217-22

    • Lee S-O et al. MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney diseases. J Clin Invest 2008; 18(11):3714-24

    • Chang SS et al. miRNA alterations inhead and neck squamous cell carcinoma. Int J Cancer 2008; 123,2791-7

    • Park CS and Tang SJ. Regulation of microRNA Expression by Induction of Bidirectional Synaptic Plasticity. J Mol Neurosci. 2008; Nov 8

    • Goff LA et al. The analysis of microRNAs in stem cells. Stem Cell Res Therap 2008; 1:141-67.

    • Chang HY et al. Microarray analysis of stem cells and their differentiation. Essential stem cell methods 2008; Chapter 12.

    • Chen X-M et al. A cellular miRNA, let-7i, regulates toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J. Biol. Chem. 2007; July 27

    • Baroukh N etc. MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta -cells lines. J Biol Chem 2007; Apr 26

    • Donker RB et al. The expression of argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Mol Hum Reprod 2007; 13(4):273-9.

    • Mott JL et al. Mir-29 regulates Mcl-1 protein expression and apoptosismer-29 downregulates Mcl-1. Oncogene 2007; 26:6133-40.

    • Weston MD et al. MicroRNA gene expression in the mouse inner ear.
    Brain Res 2006; 1111(1)95-104

    Contact us if you want to order or have more questions.

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